2017-8-11 16:0:32025-05-18 20:58:05
locus
BSU28280
BSU_28280
geneLength
1554
1557
outlinks
bsu
BSU28280
BSU_28280
Gene
Coordinates
2,892,138 → 2,893,694
2,892,138 2,893,694
The protein
Catalyzed reaction/ biological activity
Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA (according to Swiss-Prot)
3-methyl-2-oxobutanoate + acetyl-CoA + H2O --> (2S)-2-isopropylmalate + CoA + H+ (according to UniProt)
The protein
Protein family
LeuA type 1 subfamily (according to Swiss-Prot)
alpha-IPM synthase/homocitrate synthase family (single member, according to UniProt)
The protein
[SW|Localization]
cytoplasm (according to Swiss-Prot), membrane [Pubmed|18763711]
cytoplasm (according to UniProt)
membrane [Pubmed|18763711]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
Pyruvate carboxyltransferase domain (aa 4-266) (according to UniProt)
Biological materials
Mutant
BKE28280 ([[gene|leuA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCACGAAGCGTCGTATCGA, downstream forward: _UP4_TAAAAGAAAGGAGAACGGTT
BKK28280 ([[gene|leuA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCACGAAGCGTCGTATCGA, downstream forward: _UP4_TAAAAGAAAGGAGAACGGTT